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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important function in advertising and marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two supplemental splice variants were noted but specific nucleotide sequence information and facts isn't but offered.
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Blocking peptides are peptides that bind specifically into the focus on antibody and block antibody binding. These peptide typically includes the epitope recognized because of the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...
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This information outlines additional attainable optimization of mobile and immunoassay parameters to make sure the absolute best final results are...
Antibodies sure to the blocking peptide now not bind to the epitope around the goal protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is certain.
Goal Data Cyclin E belongs on the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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